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KMID : 0903520060490030227
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2006 Volume.49 No. 3 p.227 ~ p.232
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Effect of Aceton Extract from Styela Clava on Oxidative DNA Damage and Anticancer Activity
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Seo Bo-Young
Jung Eun-Sil
Kim Ju-Young
Park Hae-Ryong
Lee Seung-Cheol
Park Eun-Ju
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Abstract
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Styela clava (also called as rough sea squirt or leathery tunicate) is regarded as native to the northwest Pacific region including Korea and widely distributed in parts of northwestern Europe, North America and Australia. To evaluate Styela clava as a potential bioactive agent, the antioxidant activity of aceton extracts from Styela clava (whole, substance and tunic) was tested by measuring inhibitory effect of H2O2 induced DNA damage using comet assay. Also, anticancer activity on human colon cancer cell (HT-29) was investigated by MTT reduction assay. The 200 μM H2O2 induced DNA damage was inhibited with Styela clava aceton extract in dose dependent manner in human leukocytes. The maximum inhibition was by 62.8, 62.1 and 78.3% at the concentration of 50 μg/ml of whole, substance and tunic extracts, respectively. The aceton extracts from S. clava were also found to inhibit the growth of human colon cancer cell. The cell proliferation rates decreased to 26.9, 30.6 and 12.0% at the concentration of 500 μg/ml of whole, substance and tunic extracts, respectively. These results support that aceton extracts from S. clava may be a potential candidate as a possible antimutagenic and chemotherapeutic agent.
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KEYWORD
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Styela clava, cytotoxic effect, MTT reduction assay, DNA damage, comet assay
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